Reverse transcriptase-free detection of viral RNA using Hemo Klentaq DNA polymeraseArticle Published on 2022-12-152022-11-15 Journal: Analytical biochemistry [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] affected amplification Analysis can be used caused comparable COVID-19 Degraded RNA demonstrated determine DNA polymerase Efficiency freeze-thaw cycles limitations Nasopharyngeal swab Nearby Nearby primers overcome pandemic patients PCR polymerase chain polymerase chain reaction Primers provide provided reliability reverse transcriptase Reverse transcriptase activity reverse transcription RNA RT-PCR SARS-CoV-2 SARS-CoV-2 detection target transcriptase Viral RNA Viral RNA. [DOI] 10.1016/j.ab.2022.114960 PMC 바로가기
RT-LAMP assay for rapid detection of the R203M mutation in SARS-CoV-2 Delta variantSARS-CoV-2 Delta 변이체에서 R203M 돌연변이의 신속한 검출을 위한 RT-LAMP 분석Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] Accuracy amplification analyses appear AUC clinical samples conserved COVID-19 COVID-19 vaccines Delta delta variant Delta variants detect determined by Diagnosis discrepancy dominant Efficiency facilitate genotyping infected individuals Mutation N gene positive sample Primers quantification quarantine R203M mutation Rapid rapid diagnosis. RT-LAMP RT-qPCR SARS-CoV-2 sensitivity sequence Sequencing sets of primer sets of primers specificity specimen Surveillance threshold variant variants [DOI] 10.1080/22221751.2022.2054368 PMC 바로가기 [Article Type] Article
Development of a Loop-Mediated Isothermal Amplification Method for Rapid and Visual Detection of Monkeypox VirusArticle Published on 2022-10-262022-11-16 Journal: Microbiology Spectrum [Category] 치료기술, [키워드] amplification caused clinical sample clinical samples COVID-19 pandemic detection Detection limit development Diagnosis early stage evaluated hospital Infection isothermal isothermal amplification Loop-mediated isothermal amplification Monkeypox Monkeypox virus orthopoxvirus outbreak pathogen pathogenic virus Pathogens PCR primer set Primers public health threat Rapid rapid detection sensitivity sequence specificity Symptom temperature virus Visual visual detection. [DOI] 10.1128/spectrum.02714-22 PMC 바로가기
The Efficient and Practical virus Identification System with ENhanced Sensitivity for Solids (EPISENS-S): A rapid and cost-effective SARS-CoV-2 RNA detection method for routine wastewater surveillance고형물에 대한 감도가 향상된 효율적이고 실용적인 바이러스 식별 시스템(EPISENS-S): 일상적인 폐수 감시를 위한 빠르고 비용 효율적인 SARS-CoV-2 RNA 검출 방법Article Published on 2022-10-152022-09-11 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] applicability centrifugation city collected Concentration confirmed COVID-19 case COVID-19 demonstrated Detection sensitivity efficient Epidemiology evaluated examined followed by heat heat-inactivated identification Japan magnitude Mild multiplex normalized pellet Pepper mild mottle virus plant primer Primers qPCR qPCR quantification qPCR. Quantification method quantified ranged reported reverse primer reverse primers RNA concentration RNA concentrations RNA extraction SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection sensitivity Solid subsequent Surveillance System target targets the SARS-CoV-2 Treatment virus wastewater Wastewater-based epidemiology [DOI] 10.1016/j.scitotenv.2022.157101 PMC 바로가기 [Article Type] Article
PrimedSherlock: a tool for rapid design of highly specific CRISPR-Cas12 crRNAsArticle Published on 2022-10-142022-11-15 Journal: BMC Bioinformatics [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] assays automated benefit bioinformatics Cas12 nuclease assay Cas12a Combination conserved CRISPR CRISPR-Ca CRISPR-Cas12 Critical demonstrated Dengue diagnostic diagnostics Efficiency facilitated FDA approval field Field forward diagnostics flavivirus genomes genomic HIV In-vitro Intensive isothermal isothermal amplification manual curation Nile nucleases nucleic acid offer parameter Pathogens platform polymorphism PrimedRPA PrimedSherlock primer primer pair Primers qRT-PCR regions RNA robust SARS-CoV-2 Serological assay serotype SHERLOCK significantly specificity strain Strains target targets Vector-borne disease West West Nile wildtype Zika Zika. [DOI] 10.1186/s12859-022-04968-5 PMC 바로가기
Detection of SARS-CoV-2 in different human biofluids using the loop-mediated isothermal amplification assay: A prospective diagnostic study in Fortaleza, Brazil루프 매개 등온 증폭 분석을 사용하여 다양한 인간 생체 유체에서 SARS-CoV-2 검출: 브라질 Fortaleza의 전향적 진단 연구Article Published on 2022-09-012022-09-11 Journal: Journal of medical virology [Category] COVID19(2023년), SARS, 치료기술, [키워드] 95% CI 95% confidence interval Accuracy acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 adopted biofluid Brazil caused Chain Reaction collected coronavirus coronavirus disease Coronavirus disease 2019 COVID-19 detect detection Diagnosis diagnostic evaluated gold gold standard human gene isothermal amplification LAMP Loop-mediated isothermal amplification management Molecular diagnosis Molecular diagnosis. Nasopharyngeal swab nasopharyngeal swabs ORF1 ORF1ab oropharyngeal swabs pandemic Patient polymerase chain polymerase chain reaction primer primer set Primers processed Quantitative quantitative polymerase chain reaction RT-LAMP RT-qPCR SARS-CoV-2 Severe acute respiratory syndrome severe acute respiratory syndrome Coronavirus specificity [DOI] 10.1002/jmv.27842 PMC 바로가기 [Article Type] Article
Nasopharyngeal microbiota profiling of pregnant women with SARS-CoV-2 infectionSARS-CoV-2 감염 임산부의 비인두 미생물총 프로파일링Multicenter Study Published on 2022-08-042022-09-12 Journal: Scientific Reports [Category] SARS, 진단, [키워드] 16S rRNA acute infection Alpha amplified antibodies antibody association ASV Bacteria Bacteroidete Barcelona change COVID-19 pandemic cross-sectional diagnosed DNA enrolled evaluated Infection long-lasting microbiota Mild multicenter nasopharyngeal Nasopharyngeal swab negative RT-PCR Peripheral blood population-based cohort positive positive RT-PCR Pregnancy pregnant women Primers profile Randomly reported RT-PCR SARS-CoV-2 SARS-CoV-2 antibodies SARS-CoV-2 IgG SARS-COV-2 infection selected severe cases significant difference Spain Tenericute was determined was tested women [DOI] 10.1038/s41598-022-17542-z PMC 바로가기 [Article Type] Multicenter Study
Identification of conserved regions from 230,163 SARS-CoV-2 genomes and their use in diagnostic PCR primer design230,163 SARS-CoV-2 게놈에서 보존 영역 식별 및 진단 PCR 프라이머 디자인에서의 사용Article Published on 2022-08-012022-09-11 Journal: Genes & genomics [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] addition Alpha amplification approach Beta bioinformatics can be used candidate Candidates characteristic conserved Coverage dataset Delta Diagnosis diagnostic Epsilon Eta evaluated exhibited False negative full-length Gamma genome sequence genomes genomic sequence GISAID GISAID EpiCoV database identification identify in silico Intensive Iota Multiple Multiple sequence alignment multiple sequence alignments NCBI nine NSP2 orf3a gene Outbreaks pandemic PCR PCR primer primer primer set primer sets Primers qPCR RdRP Region removal retrieved RNA sample RNA samples RT-qPCR S gene SARS-CoV-2 SARS-CoV-2 genome SARS-CoV-2 genomes SARS-CoV-2 variant SARS-CoV-2. sequence Sequence alignment target viral genome viral sequences was reduced Zeta [DOI] 10.1007/s13258-022-01264-7 PMC 바로가기 [Article Type] Article
High throughput SARS-CoV-2 variant analysis using molecular barcodes coupled with next generation sequencing차세대 시퀀싱과 결합된 분자 바코드를 사용하여 처리량이 높은 SARS-CoV-2 변이체 분석Article Published on 2022-06-212022-09-11 Journal: PLoS ONE [Category] COVID19(2023년), MERS, SARS, 변종, 진단, [키워드] added amplifying Analysis benefit diagnostic Diagnostic method DNA epidemiological followed by globe housekeeping gene identify Illumina implication Infection Internal control Modification molecular next generation sequencing NGS nucleotide pandemic pooling positive positive sample primer Primers purified RT-PCR SARS-CoV-2 variant SARS-CoV-2 variants Screen sensitivity sequence Sequencing single nucleotide specificity vaccination variant Viral viral gene viral genes viral mutation Viral mutations virus [DOI] 10.1371/journal.pone.0253404 PMC 바로가기 [Article Type] Article
Detection of SARS-CoV-2 RNA through tandem isothermal gene amplification without reverse transcription역전사 없이 탠덤 등온 유전자 증폭을 통한 SARS-CoV-2 RNA 검출Article Published on 2022-06-152022-09-11 Journal: Analytica chimica acta [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, 치료기술, [키워드] Accuracy alternative amplification amplified Analysis cDNA complex composed COVID-19 COVID-19 epidemic Cycling detect detection diagnose Diagnosis DNA emission form G-quadruplex gene amplification generate hairpin structure isothermal Isothermal gene amplification linear primer Primers produced RCA repeated reverse transcription Rolling Rolling circle amplification SARS-COV-2 infection SARS-CoV-2 RNA SARS-CoV-2 viral SARS-CoV-2 viral RNA sensitivity single-stranded DNA ssDNA strand Strand displacement amplification subsequent Thioflavin Thioflavin T. viral genes Viral RNA [DOI] 10.1016/j.aca.2022.339909 PMC 바로가기 [Article Type] Article